肉多荤文高H羞耻玩弄校园,国产女人高潮嗷嗷嗷叫,A级毛片在线看,国产国语老龄妇女a片

熱門搜索: 肌酸激酶MB型同工酶(CK-MM)質(zhì)控樣品 抗血漿鑒別試劑盒 甲種胎兒球蛋白抗原 重組核心鏈霉親和素 2(不帶標(biāo)簽) 重組鏈霉親和素(r-ScSA)(NC膜專用) 重組鏈霉親和素(帶His標(biāo)簽) 羊抗人視-黃醇結(jié)合蛋白多克隆抗體 大鼠白細(xì)胞介素1β(IL-1β)試劑盒(ELISA) 人堿性成纖維細(xì)胞生長因子ELISA試劑盒 1%豚鼠紅細(xì)胞 2%雞紅細(xì)胞 1%雞紅細(xì)胞 重組人線粒體核糖體蛋白S2(MRPS2) 重組人蛋白酶體激活物亞基1(PSME1)蛋白 重組人肝癌衍生生長因子相關(guān)蛋白3 重組人FAM83A蛋白

PRODUCT CLASSIFICATION

產(chǎn)品分類

技術(shù)文章/ Technical Articles

您的位置:首頁  /  技術(shù)文章  /  ELISA實(shí)驗(yàn)英文介紹

ELISA實(shí)驗(yàn)英文介紹

更新時(shí)間:2013-05-07      瀏覽次數(shù):2924

ELISA (Enzyme-Linked ImmunoSorbant Assay)

ELISA實(shí)驗(yàn)和ELISA試劑盒的原理介紹(英文原版)

The purpose of an ELISA is to determine if a particular protein is present in a sample and if so, how much. There are two main variations on this method: you can determine how much antibody is in a sample, or you can determine how much protein is bound by an antibody. The distinction is whether you are trying to quantify an antibody or some other protein. In this example, we will use an ELISA to determine how much of a particular antibody is present in an individuals blood.

ELISAs are performed in 96-well plates which permits high throughput results. The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

After some time, the serum is removed and weakly adherent antibodies are washed off with a series of buffer rinses. To detect the bound antibodies, a secondary antibody is added to each well. The secondary antibody would bind to all human antibodies and is typically produced in a rodent. Attached to the secondary antibody is an enzyme such as peroxidase or alkaline phosphatase. These enzymes can metabolize colorless substrates (sometimes called chromagens) into colored products. After an incubation period, the secondary antibody solution is removed and loosely adherent ones are washed off as before. The final step is the addition the enzyme substrate and the production of colored product in wells with secondary antibodies bound.

When the enzyme reaction is complete, the entire plate is placed into a plate reader and the optical density (i.e. the amount of colored product) is determined for each well. The amount of color produced is proportional to the amount of primary antibody bound to the proteins on the bottom of the wells.

微信掃一掃

郵箱:1170233632@qq.com

傳真:021-51870610

地址:上海市顧戴路2988號(hào)B幢7樓

Copyright © 2025 上海信帆生物科技有限公司版權(quán)所有   備案號(hào):滬ICP備13019554號(hào)-1    技術(shù)支持:化工儀器網(wǎng)

TEL:13764793648

掃碼加微信
精品少妇人妻AV一区二区| 亚洲精品国产精华液| 亚洲国产精品99久久久久久| 丰满女人又爽又紧又丰满| 国产熟女一区二区三区五月婷| 熟女精品视频一区二区三区| 少妇被粗大猛进进出出s小说| 少妇荡乳情欲办公室| 国产V亚洲V天堂无码| 国产精品9999久久久久仙踪林| 日日碰狠狠躁久久躁| 成免费CRM直接看| 把极品白丝班长啪到腿软| FREE性开放小少妇| 艳妇500篇短篇H系列| 大秦赋全集1-78集免费观看 | 极品粉嫩国产18尤物在线播放| 高H猛烈失禁潮喷A片在线观看| 被下人玩弄的嫡女NP| 浴室人妻的情欲HD三级国产| 阿娇13分钟视频无删减MP4| 久久久久久久精品免费久精品蜜桃| 闺房床榻粗喘H娇女| 巨粗进入警花哭喊求饶| 亚洲精品97久久中文字幕无码| 亚洲AV天堂AV在线成人播放| 男男潮喷翻白眼漏尿高H漫画| 双性喂奶给室友八人攻| 被多个强壮黑人灌满精H| 最近最新的日本字幕MV| 边亲边脱边捏胸视频| 亚洲午夜精品久久久久久浪潮| 妻子3免费完整版hd| 情欲情欲欲超市全文无删减| 免费男女囗交视频在线观看| JAPANESE熟睡侵犯| 久久女婷五月综合色啪小说| 欧美在线 | 亚洲| 性疼痛TUBE小坳交HD| 成AV免费大片黄在线观看| 无码国产69精品久久久孕妇|