【產(chǎn)品中文名稱】 | 人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA kit |
【產(chǎn)品英文名稱】 | Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA kit | | | |
【保存溫度】 | 2-8℃,避光保存 | | | |
【產(chǎn)品規(guī)格】 | 96T/48T | | | |
【有效期】 | 6個(gè)月 | | | |
購(gòu)買本司試劑盒,全程提供技術(shù)指導(dǎo),有質(zhì)量問(wèn)題免費(fèi)包退換.提供免費(fèi)代測(cè)服務(wù),歡迎. | |
該試劑盒適合檢測(cè)血清,血漿,細(xì)胞上清,組織等樣本,產(chǎn)品靈敏度高,具體見(jiàn)產(chǎn)品說(shuō)明書(shū). | |
如果你需要試劑盒的樣本收集處理資料以及試劑盒說(shuō)明書(shū),索要! | |
人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA kit | | | |
【注意事項(xiàng)】 | | | | |
1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。 |
2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。 | |
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。 | | |
一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。 | |
4.請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值), |
請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)zui后乘以總稀釋倍數(shù)(×n×5)。 | |
人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA kit |
【實(shí)驗(yàn)中zui可能出現(xiàn)問(wèn)題】 | | | |
○一 所有孔未顯色 ? 分析原因: | |
(1) 微孔加入抗體后,洗板不*,微孔中存在游離的抗體。 | | | |
(2)用錯(cuò)試劑。 | | | |
(3) 在操作中忘記加酶或抗體。 ? | | | |
解決辦法: | | | | |
(1) 檢查洗板器械,比如洗板機(jī)管道是否堵塞、洗液是否用完。 | | | |
(2)在加樣前,要看清瓶簽。 | | | |
(3) 嚴(yán)格按說(shuō)明書(shū)操作步驟進(jìn)行實(shí)驗(yàn),確保每步都到位。 | | | |
○二 所有孔顯色偏低 ? 分析原因: |
(1)微孔加入抗體后,洗板不*,微孔中存在游離的抗體。 | | | |
(2) 洗板不干凈。 | | | |
(3) 抗體污染了酶結(jié)合物等其它試劑。 | | | |
(4)溫度沒(méi)達(dá)到要求。 | | | |
(5) 每孔加入的試劑量低于說(shuō)明書(shū)要求量。 ? | | | |
2.解決辦法: | |
(1)手工加蒸餾水洗板時(shí),要避免吸頭接觸微孔,常更換新的蒸餾水。 |
(2) 洗板時(shí)要確保每孔都注滿蒸餾水(約400ul),且至少洗板五次。 | |
(3)嚴(yán)格按說(shuō)明書(shū)要求操作,每次取樣要更換吸頭。 | |
(4) 控制孵育溫度在25℃。 | | |
(5) 加樣器吸頭安放牢固避免吸樣不足,加樣時(shí)務(wù)必將吸頭內(nèi)液體打盡。 | |
人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA kit | | | |
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